The mycobiont and whole thallus cultures of Bel. were established using soredia as an inoculum.The mycobiont Roccella montagnei cultures showed optimum growth, biomass and biosynthesis of compounds in Lilly and Barnett medium with glucose as a carbon source, micronutrients and vitamins. After the incubation period of 180 days, the cultures were harvested, and their biomass and secondary compound profiles were analysed. The HPTLC chromatogram of the acetone extract of erythrin the NT and...
T-homoeolog specific plasma membrane protein 3 [Nt(t)PMP3-2] in polyploid Nicotiana tabacum shows conserved alternative splicing, derived from extant Nicotiana tomentosiformis parent
Abiotic stress induced plasma membrane protein 3 (PMP3) genes occur as multigene families in plants, coding for hydrophobic proteins. Group I PMP3s code for shorter ORFs while Group II PMP3s code for proteins with C-terminal extensions. Allotetraploid Nicotiana tabacum (SSTT; 2n = 48) derives its parentage from extant ancestors related to Nicotiana sylvestris (SS) and Nicotiana tomentosiformis (TT). Polyploidization triggers complex genetic and epigenetic changes, often leading to homoeolog-...
THREE DECADES OF EXPERIENCE WITH GENETICALLY ENGINEERED CROPS: WAY FORWARD IN THE INDIAN CONTEXT
An important outcome of the elucidation of the double helix structure of DNA is the beginning of the science of genetic engineering. Genetic engineering has made it possible to transfer genes across
sexual barriers. However there are several of environmental, biosafety and other problems associated with the technology. Therefore, we must have a very good mechanism for measuring risks and benefits. The report
submitted to the Government over ten years ago, one of us (MSS) had pointed out that, “...
Evaluation of the genetic diversity and population structure in drumstick (Moringa oleifera L.) using SSR markers
Moringa belongs to the family Moringaceae comprising 13 species of which Moringa oleifera is more widely
cultivated. It is an economically important multipurpose tree with immense nutritional value and
has significant potential to address malnutrition. In the present study, a total of 97 accessions collected
from different districts of Tamil Nadu, Andhra Pradesh and Odisha were genotyped using 20 simple
sequence repeat (SSR) markers to assess the genetic diversity and population structure. A...
Microbial Consortial Products for Sustainable Agriculture: Commercialization and Regulatory Issues in India
Rhizosphere microorganisms directly and indirectly influence the composition and productivity of natural plant communities. Hence, belowground microbial species richness has been proposed as a predictor of aboveground plant diversity and productivity. Though research-based evidences clearly show the advantages of microbial consortia-based products due to their multifunctionality, limited attention is being given to develop quality standards for registration. This chapter focuses on the uses,...
Sustaining and enhancing crop productivity in an era of climate change
The earth is experiencing a faster change in climate in
the 21st century than it had in the past. Abiotic
stresses such as drought and salinity, exacerbated by
the fast changing climatic conditions pose a major
hurdle in sustaining crop productivity. Developing
crop plants able to yield better under abiotic stresses
offer hope in this situation. Understanding abiotic
stress-tolerance mechanisms in a plant system is crucial
to improve the stress tolerance. The present
review discusses broad...
Characterization of the predominant bacterial population of different mangrove rhizosphere soils using 16S rRNA gene-based single-strand conformation polymorphism (SSCP)
Variations in chemical parameters and bacterial populations in mangrove rhizosphere samples were noted for different sites. The C, N, P and K contents as well as pH, EC and salinity showed variation between sites. Significant differences in soil properties were also found in sampling sites. Two types of soil were noted among sites. Guesthouse had significantly higher organic matter and nutrient content (N) than other three sites suggesting that human discharges, litter deposition and surface...
Diversity and N-acyl-homoserine lactone production by Gammaproteobacteria associated with Avicennia marina rhizosphere of South Indian mangroves
Thediversity of N-acyl-homoserine lactone (AHL)-producing rhizosphere bacterial community associated with Avicennia marina in the mangrove ecosystems of South India was investigated. Approximately 800 rhizobacteria were isolated from A. marina, and they were screened for the production of AHL using two biosensors, Chromobacterium violaceum CV026 and Agrobacterium tumefaciens NTL4 (pZLR4). Among the total isolates screened, 7% of the rhizobacteria showed positive induction for AHL signals. The...
A significant proportion of indigenous rhizobia from India associated with soybean (Glycine max L.) distinctly belong to Bradyrhizobium and Ensifer genera
The diversity among 269 rhizobia isolated from naturally occurring root nodules of soybean collected from
two different agro-ecological regions of India, based on RFLP and sequences of the intergenic spacer (IGS)
between the 16S and 23S rRNA genes, growth rate, and indole acetic acid production, revealed their significant,
site-dependent genomic diversity. Among these bacteria, nine IGS genotypes were identified with two endonucleases. They were distributed into five divergent lineages by...