Diversity and N-acyl-homoserine lactone production by Gammaproteobacteria associated with Avicennia marina rhizosphere of South Indian mangroves
Thediversity of N-acyl-homoserine lactone (AHL)-producing rhizosphere bacterial community associated with Avicennia marina in the mangrove ecosystems of South India was investigated. Approximately 800 rhizobacteria were isolated from A. marina, and they were screened for the production of AHL using two biosensors, Chromobacterium violaceum CV026 and Agrobacterium tumefaciens NTL4 (pZLR4). Among the total isolates screened, 7% of the rhizobacteria showed positive induction for AHL signals. The...
A significant proportion of indigenous rhizobia from India associated with soybean (Glycine max L.) distinctly belong to Bradyrhizobium and Ensifer genera
The diversity among 269 rhizobia isolated from naturally occurring root nodules of soybean collected from
two different agro-ecological regions of India, based on RFLP and sequences of the intergenic spacer (IGS)
between the 16S and 23S rRNA genes, growth rate, and indole acetic acid production, revealed their significant,
site-dependent genomic diversity. Among these bacteria, nine IGS genotypes were identified with two endonucleases. They were distributed into five divergent lineages by...
Ciceribacter lividus gen. nov., sp. nov., isolated from rhizosphere soil of chick pea ( Cicerarietinum L.)
The taxonomic position of strain MSSRFBL1T
, isolated from chickpea rhizosphere soil from
Kannivadi, India, was determined. Strain MSSRFBL1T formed bluish black colonies, stained
Gram-negative and was motile, aerobic, capable of fixing dinitrogen, oxidase-negative and
catalase-positive. Q-10 was the major respiratory quinone. Major fatty acids of strain
MSSRFBL1T were C18 : 1v7c and C19 : 0cyclov8c. Minor amounts of C18 : 0, C12 : 0, C14 : 0 3-OH,
C18 : 0 3-OH, C16 : 0, C16 : 1v6c/C16 : 1v7c,...
Cloning and Characterization of a Gene Encoding Ubiquitin Conjugating Enzyme from the Mangrove Species, Avicennia marina (Forsk.) Vierh.
Covalent attachment of ubiquitin has been implicated in mediating proteolysis of the cellular proteins by Ubiquitin- proteasome pathway. Ubiquitin activating enzyme (E1), ubiquitin conjugating enzyme (E2), and ubiquitin protein ligase (E3) are the three enzymes involved in this process. This paper reports the isolation of a gene that codes for the ubiquitin conjugating enzyme in Avicennia marina (AmUBC2), and regulation of its expression at RNA level under salt stress. Deduced amino acid...
Magnitude of hybrid vigor retained in double haploid lines of some heterotic rice hybrids
Sixty one double haploid (DH) lines were evaluated
for yield components and yield and compared with
the three corresponding hybrids from which they were derived
through anther culture. Analysis of a 6x6 diallel experiment
led to the selection of these hybrids based on their
high heterosis and revealed the nature of gene action of the
characters under evaluation. The DH lines along with the
hybrids and parents were planted following a simple lattice
design with two replications. The results show...
Ploidy-depdenten genomic stability in the tissue cultures of ornamental Phlox drummondii Hook
Comparisons of the chromosome numbers, 2C nuclear DNA amounts and karyomorphology were made in explant cultures of diploid (2n = 2,x = 14) and autotetraploid (2n = 4x = 28) Phlox drummondii. In 6-36 week old calli derived from diploid intemodai segment explants, and in cells of root tips regenerated from such callus, marked differences were observed in chromosome number. The chromosome numbers ranged from 2n = 14 to 2n = 100 and DNA amounts from 8.20 to 63.20 pg in the diploid derived callus,...
Recognition and reward to the tribal and farming communities for conservation of agro-biodiversity*
India, in compliance with the requirement under the Trade Related aspects of Intellectual Property Rights (TRIPS) of the World Trade Organization (WTO), enacted the Protection of Plant Varieties and Farmers’ Rights Act (PPVFR Act) in 2001. TRIPS require Member countries of WTO to provide legal protection to plant varieties either by patents or by an effective sui generis system or a combination of both1 . Having India chosen not to grant patent to plants and animals and the parts thereof,...
Identification of a sex-specific SCAR marker in dioecious Pandanus fascicularis L. (Pandanaceae).
Pandanus fascicularis L. is a dioecious plant native to South Asia with significant numbers in coastal areas. With the aim of distinguishing male genotypes from female genotypes early in the vegetative growth phase, the current study was initiated using molecular markers. Based on the principle of bulked segregant analysis, the sampled plants were separated into 2 bulks depending on their sex. Of the 89 random amplified polymorphic DNA and inter-simple sequence repeat markers used, one decamer...
Analysis of Species Relationship Among Seven Small Millets Using Molecular Markers
Genomic DNA of twenty four accessions belonging to seven small millet species were analyzed for RAPD, RFLP and AFLP profiles for a comprehensive understanding of the level of genetic diversity within the species and relationships between them. Thirty random primers generated a total of 115 amplification products of which 70 were polymorphic across all species.Twenty-five probe enzyme combinations were used for RFLP analysis that revealed 87 loci of which 62 were polymorphic across the species....