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Research Publication

  • A salt-inducible chloroplastic monodehydroascorbate reductase from halophyte Avicennia marina confers salt stress tolerance on transgenic plants

    Plant growth and productivity are adversely affected by various abiotic stress factors. In our previous study, we used Avicennia marina, a halophytic mangrove, as a model plant system for isolating genes functioning in salt stress tolerance. A large scale random EST sequencing from a salt stressed leaf tissue cDNA library of one month old A. marina plants resulted in identification of a clone showing maximum homology to Monodehydroascorbate reductase (Am-MDAR). MDAR plays a key role in...

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  • Genetic diversity in cultivars and landraces of Oryza sativa subsp. indica as revealed by AFLP markers

    Genetic diversity among 49 Indian accessions of rice (Oryza sativa subsp. indica), including 29 landraces from Jeypore, 12 modern cultivars, and 8 traditional cultivars from Tamil Nadu, was investigated using AFLP markers. In total, nine primer combinations revealed 664 AFLPs, 408 of which were found to be polymorphic. The percentage of polymorphic AFLPs was approximately the same within the cultivars and landraces. Similar results were obtained when genetic diversity values were estimated...

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  • A chloroplast-localized and auxin-induced glutathione S-transferase from phreatophyte Prosopis juliflora confer drought tolerance on tobacco

    Plant growth and productivity are adversely affected by various abiotic stress factors. In our previous study, we used Prosopis juliflora, a drought-tolerant tree species of Fabaceae, as a model plant system for mining genes functioning in abiotic stress tolerance. Large-scale random EST sequencing from a cDNA library obtained from drought-stressed leaves of 2-month-old P. juliflora plants resulted in identification of three different auxin-inducible glutathione S-transferases. In this paper,...

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  • Species identification in seven small millet species using polymerase chain reaction – restriction fragment length polymorphism of trnS-psbC gene region

    The chloroplast trnS-psbC gene regions from total genomic DNA of 119 accessions from seven small millet species were amplified by polymerase chain reaction (PCR) and digested with eight restriction enzymes individually as well as in combinations of two enzymes to generate restriction fragment length polymorphism (PCR–RFLP). PCR– RFLP with individual enzymes revealed polymorphism between only some species. However, all the species could be distinguished by using a combination of two enzymes,...

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  • A diurnally regulated dehydrin from Avicennia marina that shows nucleo-cytoplasmic localization and is phosphorylated by Casein kinase II in vitro

    Dehydrins have a key role in protecting plants from dehydration stress. We report here the isolation of two cDNAs coding for the same dehydrin, AmDHN1 and AmDHN1a from salt stressed leaves of Avicennia marina (Forsk.) Vierh. by EST library screening. AmDHN1 was found to contain a retained intron that was absent in AmDHN1a. AmDHN1 expression in the context of various environmental stresses was investigated. In leaves, AmDHN1 shows a diurnal pattern of regulation and is induced only by mannitol...

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  • Capacity Building is the Key to Achieving Appropriate Biosafety Protocols

    Over the last two centuries, science, technology and public policy have played a significant role in terms of helping farmers to increase agricultural productivity. Today, we are entering the new millennium with a number of advanced tools at hand. Among them, biotechnology is of major importance. The progress in the field of molecular biology, microbiology, and genetic engineering in the last two decades has been enormous.

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  • Isolation and Characterization of an Atypical LEA Protein Coding cDNA and its Promoter from Drought-Tolerant Plant Prosopis juliflora

    Plant growth and productivity are adversely affected by various abiotic and biotic stress factors. Despite the wealth of information on abiotic stress and stress tolerance in plants, many aspects still remain unclear. Prosopis juliflora is a hardy plant reported to be tolerant to drought, salinity, extremes of soil pH, and heavy metal stress. In this paper, we report the isolation and characterization of the complementary DNA clone for an atypical late embryogenesis abundant (LEA) protein (Pj...

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  • The Electronic Plant Gene Register

    Nucleotide Sequence of a Truncated cDNA (Accession No. AF093108) Encoding a H3-Like Histone Protein from the Bryophyte Tortula ruralis. R. Joel Duff, Melvin J. Oliver, and Andrew J. Wood*. Department of Plant Biology, Southern Illinois University- Carbondale, Carbondale, Illinois 62901–6509 (R.J.D., A.J.W.); and Plant Stress and Water Conservation Unit, United States Department of Agriculture-Agricultural Research Service, Route 3, Box 215, Lubbock, Texas 79401 (M.J.O.).

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  • Characterization of a non-reducing polyketide synthase gene from lichen Dirinaria applanata

    Lichens are known to produce a variety of secondary metabolites including polyketides that have diverse biological role(s). The biosynthesis of fungal polyketides is governed by type I polyketide synthases (PKS), enzymes with a multidomain structure, including the b-ketoacyl synthase (KS), acyl transferase (AT), ketoreductase (KR), dehydratase (DH), enoyl reductase (ER) and acyl carrier protein (ACP) domains. Established soredial cultures of Dirinaria applanata (Fée) producing atranorin and...

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  • Molecular phylogeny of mangroves 11. Intraand inter-specif ic variation in Avicennia revealed by RAPD and RFLP markers

    Random amplified polymorphic DNA (RAPD) and restriction fragment length polymorphism (RFLP) markers were used to estimate intra- and inter-specific variation in three species of an exclusive mangrove genus, Avicennia. Intrapopulation polymorphism among the 10 populations of Avicennia marina, as measured by percentage of polymorphic RAPDs, varied between 17.8 and 38.9%, with a standard deviation of 7.28, and the coefficient of variation was 26.5%. Polymorphism in Avicennia ofJicinalis (...

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