Isolation and Characterization of an Atypical LEA Protein Coding cDNA and its Promoter from Drought-Tolerant Plant Prosopis juliflora
Plant growth and productivity are adversely affected by various abiotic and biotic stress factors. Despite the wealth of information on abiotic stress and stress tolerance in plants, many aspects still remain unclear. Prosopis juliflora is a hardy plant reported to be tolerant to drought, salinity, extremes of soil pH, and heavy metal stress. In this paper, we report the isolation and characterization of the complementary DNA clone for an atypical late embryogenesis abundant (LEA) protein (Pj...
Nucleotide Sequence of a Truncated cDNA (Accession No. AF093108) Encoding a H3-Like Histone Protein from the Bryophyte Tortula ruralis. R. Joel Duff, Melvin J. Oliver, and Andrew J. Wood*. Department of Plant Biology, Southern Illinois University- Carbondale, Carbondale, Illinois 62901–6509 (R.J.D., A.J.W.); and Plant Stress and Water Conservation Unit, United States Department of Agriculture-Agricultural Research Service, Route 3, Box 215, Lubbock, Texas 79401 (M.J.O.).
Characterization of a non-reducing polyketide synthase gene from lichen Dirinaria applanata
Lichens are known to produce a variety of secondary metabolites including polyketides that have diverse biological role(s). The biosynthesis of fungal polyketides is governed by type I polyketide synthases (PKS),
enzymes with a multidomain structure, including the b-ketoacyl synthase (KS), acyl transferase (AT),
ketoreductase (KR), dehydratase (DH), enoyl reductase (ER) and acyl carrier protein (ACP) domains.
Established soredial cultures of Dirinaria applanata (Fée) producing atranorin and...
Molecular phylogeny of mangroves 11. Intraand inter-specif ic variation in Avicennia revealed by RAPD and RFLP markers
Random amplified polymorphic DNA (RAPD) and restriction fragment length polymorphism (RFLP) markers were
used to estimate intra- and inter-specific variation in three species of an exclusive mangrove genus, Avicennia. Intrapopulation
polymorphism among the 10 populations of Avicennia marina, as measured by percentage of polymorphic RAPDs, varied
between 17.8 and 38.9%, with a standard deviation of 7.28, and the coefficient of variation was 26.5%. Polymorphism in
Avicennia ofJicinalis (...
Isolation and characterization of photosystem 2 PsbR gene and its promoter from drought-tolerant plant Prosopis juliflora
Prosopis juliflora is a hardy plant tolerant to drought, salinity, extremes of soil pH, and heavy metal stress. We isolated and characterized a photosystem 2 (PS2) gene PsbR (Pj PsbR) and its promoter. Northern analysis for Pj PsbR in P. juliflora leaves under 25 % polyethylene glycol stress showed steady decrease in transcript level at 12, 24, and 48 h after stress application. Under 90 mM H2O2 stress, transcript level dropped drastically at 12 h, but increased again compared to the control at...
Species Status and Relationship Between Roccella montagnei and Roccella belangeriana Using DNA Sequence Data of Nuclear Ribosomal Internal Transcribed Spacer Region
Roccella montagnei Bél emend Awas and R. belangeriana Awas were keyed out as distinct species based on their reproductive strategies. These two species are similar in their morphology and secondary chemistry. The phylogenetic relationship analysis using maximum parsimony method based on ITS sequences of the nuclear ribosomal DNA clustered these two species in a single clade with a bootstrap value of 100 indicating that these two species are the same.
ISSR and RAPD Markers Assessed Genetic Variation of Aerides maculosum – an Epiphytic Orchid from Goa, India
Aerides maculosum Lindl is one of the most important orchids valued for its beautiful inflorescence/flowers. The present study aimed to understand the level of genetic variation among the populations of A. maculosum using RAPD and ISSR markers. Among the 35 primers tested, 13 RAPD and 6 ISSR primers were selected for the analysis. Total of 101 RAPD and 40 ISSR fragments were generated. High level of polymorphism was recorded in RAPD (90.45%) compared to ISSR (72.85 %). Nei’s average genetic...
NATURE OF POLYPLOIDY IN SOME 48-CHROMOSOME SPECIES OF THE GENUS SOLANUM, SECTION TUBERARIUM
HROMOSOME numbers have been reported for 116 species in the section Tuberarium of the genus Solanum. An euploid series with 24, 36, 48, 60 and 72 somatic chromosomes exists. PRAKKEanNd SWAMINATH(1A95N2 ) concluded from the available evidence that the basic chromosome number in the genus is 12 and not 6 as suggested by some authors. Species with 48 chromosomes will, on this basis, be tetraploids and tetraploid species are present in the taxonomic series Acaulia Juz., Conicibaccata Bitt.,...
Molecular marker-based characterization in candidate plus trees of Pongamia pinnata, a potential biodiesel legume
Pongamia pinnata, a legume tree, has many traditional uses and is a potential biodiesel plant.
Despite its importance and the availability of appropriate molecular genetic tools, the full
potential of Pongamia is yet to be realized. The objective of this study was to assess
genetic diversity among 10 systematically characterized candidate plus trees (CPTs) of
P. pinnata from North Guwahati.